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DISCPLINES: Embryology Andrology Ultrasound Endocrinology
Modules HEB HEC HEE HEF HER HES HET HOva
 
Information on QA Scheme: Pronuclear Assessment-2023
Comment = A scheme to QA the assessment status of day 1 human embryos using the modified criteria described by Scott(modified). The Z score used by Scott has been expanded to generate a normally distributed value for QA purposes.
Number of enrollments =58 [* This indicates only the number of enrollments. Not all participants will have completed the scheme]
List of QA Issues (or Instalments)
Code Title Available from
HEB2023.01 Single pronuclear embryo. 1/1/2023
HEB2023.02 Single pronuclear embryo. 1/2/2023
HEB2023.03 Single pronuclear embryo. 1/3/2023
HEB2023.04 Single pronuclear embryo. 1/4/2023
HEB2023.05 Single pronuclear embryo. 1/5/2023
HEB2023.06 Single pronuclear embryo. 1/6/2023
HEB2023.07 Single pronuclear embryo. 1/7/2023
HEB2023.08 Single pronuclear embryo. 1/8/2023
HEB2023.09 Single pronuclear embryo. 1/9/2023
HEB2023.10 Single pronuclear embryo. 1/10/2023
HEB2023.11 Single pronuclear embryo. 1/11/2023
HEB2023.12 Single pronuclear embryo. 1/12/2023
List of Questions asked for QA Scheme:Pronuclear Assessment-2023
ID Question Question Format
401 Cytoplasmic Inclusions Defined Options
209 Define the pronuclear grade using Scotts Definition [HR-15(11);2394-2403). Defined Options
392 Halo effect Defined Options
389 How many pronuclei are there? Defined Options
390 Juxtaposition of pronuclei Defined Options
400 Nucleoli Location Defined Options
398 Nucleoli Number Comparison Defined Options
396 Nucleoli Number in PN#1 ( on left) Defined Options
397 Nucleoli Number in PN#2 ( on right) Defined Options
399 Nucleoli Size Defined Options
395 Nucleoli Synchrony Defined Options
391 Size of pronuclei Defined Options
693 What is the ESHRE/Alpha Pronuclei Category Defined Options
394 What is the pronuclear score for this zygote? Calculation Outcome
This question has been used in the following computations. - See Calculated Q for more details
Pronuclear Score-Complex
Detailed Information on Question: What is the pronuclear score for this zygote?
Status This is the KEY QAP Question for the Scheme    
Type Calculation Outcome
Order Question Options
1 The cummulative score is:

Title Pronuclear Score-Complex
Comment This score uses Scotts description plus other observations to create a numeric Z score for QA purposes
This is a calculated Question. It requires a number of other questions to be completed. The answer to these questions have each been weighted (see below). The value submitted for each question is then compiled as detailed below to provide a SCORE usually out of 100. The higher the score usually indicates a better quality. The score is an arbitary one and has been generated within QAPonline to allow all the parameters embodies in the questions to be assess.
Order Question
Calculation
Summation
1 Size of pronuclei
Value *1
+
2 Juxtaposition of pronuclei
Value *1
+
3 Nucleoli Synchrony
Value *1
+
4 Nucleoli Number in PN#1 ( on left)
Value *1
+
5 Nucleoli Number in PN#2 ( on right)
Value *1
+
6 Nucleoli Number Comparison
Value *1
+
7 Nucleoli Size
Value *1
+
8 Nucleoli Location
Value *1
+
9 Halo effect
Value *1
+
10 Cytoplasmic Inclusions
Value *1
+

1 Size of pronuclei
Order Option
Value
Max value
1 Equal and mature (large)
10
10
2 Unequal but mature (large)
5
10
3 Equal but Immature (small).
3
10
4 Unequal in size and maturity
1
10

2 Juxtaposition of pronuclei
Order Option
Value
Max value
1 Strongly together
10
10
2 Close or nearby
5
10
3 Not in contact - Disjuncted
2
10
4 Only one pronucleus present
0
10

3 Nucleoli Synchrony
Order Option
Value
Max value
1 Nucleoli synchronous - size, number and alignment similar
10
10
2 Nucleoli asynchronous - size, number and alignment dissimilar
5
10

4 Nucleoli Number in PN#1 ( on left)
Order Option
Value
Max value
1 <3 nucleoli
1
10
2 3-7 nucleoli
10
10
3 >7 nucleoli
1
10

5 Nucleoli Number in PN#2 ( on right)
Order Option
Value
Max value
1 <3 nucleoli
1
10
2 3-7 nucleoli
10
10
3 >7 nucleoli
1
10

6 Nucleoli Number Comparison
Order Option
Value
Max value
1 The number of nucleoli in each PN the same.
10
10
2 The number of nucleoli in each PN differ by one.
5
10
3 The number of nucleoli in each PN differ by more than one
1
10

7 Nucleoli Size
Order Option
Value
Max value
1 Nucleoli normal in size.
10
10
2 Nucleoli in at least one PN enlarged
5
10
3 Nucleoli in least one PN pinpoint - small
1
10

8 Nucleoli Location
Order Option
Value
Max value
1 Aligned along pronuclear junction
10
10
2 Starting to align along pronuclear junction
5
10
3 Scattered - Not aligned along pronucelar junction
1
10

9 Halo effect
Order Option
Value
Max value
1 Normal- slight cytoplasmic contraction
10
10
2 Light- significant cytoplasmic contraction
5
10
3 Extreme - intense cytoplamic contraction
2
10
4 No halo effect - no cytoplasmic contraction.
1
10

10 Cytoplasmic Inclusions
Order Option
Value
Max value
1 There are no inclusions in the cytoplasm
10
10
2 There are minor inclusion or vacuoles
5
10
3 There are significant inclusion or vacuoles
1
10


 
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