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Survey: pH Measurement in the IVF laboratory
Background
Recent discussions in EmbryoMail have centred around the measurement of pH. Should you use a CO2 monitor or a pH meter? This survey seeks to canvass the current status of pH measurement in the IVF laboratory.
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1. The Question: Do you regularly measure the CO2 concentration in each CO2 incubator -Daily? -Results [n=46] Data=[Results: Yes= 21 No: 25 Total= 46] ANALYSIS
2. The Question: Do you regularly measure the CO2 concentration in each CO2 incubator -Weekly? -Results [n=37] Data=[Results: Yes= 19 No: 18 Total= 37] ANALYSIS
3. The Question: Do you regularly measure the CO2 concentration in each CO2 incubator -Monthly? -Results [n=32] Data=[Results: Yes= 10 No: 22 Total= 32] ANALYSIS
4. The Question: Do you regularly measure the CO2 concentration in each CO2 incubator -More than Monthly or Never? -Results [n=23] Data=[Results: Yes= 9 No: 14 Total= 23] ANALYSIS
5. The Question: Do you regularly measure the pH by pH meter in each CO2 incubator -Daily? -Results [n=36] Data=[Results: Yes= 9 No: 27 Total= 36] ANALYSIS
6. The Question: Do you regularly measure the pH by pH meter in each CO2 incubator -weekly? -Results [n=34] Data=[Results: Yes= 8 No: 26 Total= 34] ANALYSIS
7. The Question: Do you regularly measure the pH by pH meter in each CO2 incubator -Monthly? -Results [n=34] Data=[Results: Yes= 9 No: 25 Total= 34] ANALYSIS
8. The Question: Do you regularly measure the pH by pH meter in each CO2 incubator -Never or hardly ever. ? -Results [n=30] Data=[Results: Yes= 7 No: 23 Total= 30] ANALYSIS
9. The Question: Do you pre-mixed gas for you main embryology culture -Results [n=34] Data=[Results: Yes= 9 No: 25 Total= 34] ANALYSIS
10. The Question: Do you use any visual checks for pH eg. media with phenol red dyes? -Results [n=35] Data=[Results: Yes= 12 No: 23 Total= 35] ANALYSIS
11. The Question: If you work in an laboratory higher than sea level, do you adjust your CO2 concentration? -Results [n=21] Data=[Results: Yes= 7 No: 14 Total= 21] ANALYSIS
12. The Question: Do you use sequential media? -Results [n=35] Data=[Results: Yes= 16 No: 19 Total= 35] ANALYSIS
13. The Question: Do you use global media or other single stage media? -Results [n=35] Data=[Results: Yes= 16 No: 19 Total= 35] ANALYSIS
13. The Question: Are your incubators checked at least annually? -Results [n=37] Data=[Results: Yes= 28 No: 9 Total= 37] ANALYSIS
14. The Question: If yes does this include a CO2 check? -Results [n=33] Data=[Results: Yes= 23 No: 10 Total= 33] ANALYSIS
15. The Question: Lastly do you follow the manufacturers recommendations for CO2 concentration? -Results [n=35] Data=[Results: Yes= 12 No: 23 Total= 35] ANALYSIS
Comments
#26 of . writes: This survey assumes everyone uses a pH meter to monitor pH. We use a blood-gas machine. Much more accurate, sensitive, repeatable compared to pH meter.
 
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