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Survey: Liquid Nitrogen Dewar Survey
Background
This survey explores how long you continue to use LN dewars and how you manage and audit their contents.
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Survey has been filtered by All replies from all countries
The Question was:Length of Use - Total yes replies= 85
1. The Option: LN dewars should only be used for the 10 year warranty [Results: Yes= 21 No: 64 Total= 85] ANALYSIS
2. The Option: LN dewars can be used safely for more than 12 years [Results: Yes= 41 No: 44 Total= 85] ANALYSIS
3. The Option: LN dewars can be used safely for more than15 years [Results: Yes= 23 No: 62 Total= 85] ANALYSIS
The Question was:Time between Audits - Total yes replies= 78
4. The Option: Dewars should be audited annually? [Results: Yes= 35 No: 43 Total= 78] ANALYSIS
5. The Option: Dewars should be audited every two years? [Results: Yes= 25 No: 53 Total= 78] ANALYSIS
6. The Option: Dewars can be audited more than every two years apart? [Results: Yes= 18 No: 60 Total= 78] ANALYSIS
6. The Question: Infectious and non-infectious material should be kept separate? -Results [n=54] Data=[Results: Yes= 49 No: 5 Total= 54] ANALYSIS
The Question was:Type of Audit - Total yes replies= 50
7. The Option: Just the cane location but not the number of straws need to be audited? [Results: Yes= 10 No: 40 Total= 50] ANALYSIS
8. The Option: Cane location AND the number of straws should both be audited? [Results: Yes= 40 No: 10 Total= 50] ANALYSIS
The Question was:Separation of Gametes - Total yes replies= 47
9. The Option: Sperm and embryos should be kept separate? [Results: Yes= 47 No: 0 Total= 47] ANALYSIS
10. The Option: Sperm and embryos do not need to be kept separate? [Results: Yes= 0 No: 47 Total= 47] ANALYSIS
The Question was:Separation by Method - Total yes replies= 21
11. The Option: Vitrification and slow freeze straws should be kept separate? [Results: Yes= 21 No: 0 Total= 21] ANALYSIS
12. The Option: Vitrification and slow freeze straws do not need to be kept separate? [Results: Yes= 0 No: 21 Total= 21] ANALYSIS
13. The Question: Vapour freezing must be used for infectious samples? -Results [n=47] Data=[Results: Yes= 11 No: 36 Total= 47] ANALYSIS
The Question was:Testing Patients - Total yes replies= 51
13. The Option: Patients should be tested for HIV, Hep B&C before freezing? [Results: Yes= 51 No: 0 Total= 51] ANALYSIS
14. The Option: Patients do not need be tested for HIV, Hep B&C before freezing but untested samples should be stored separately? [Results: Yes= 0 No: 51 Total= 51] ANALYSIS
15. The Option: Patients do not need to be tested for HIV, Hep B&C before freezing? [Results: Yes= 0 No: 51 Total= 51] ANALYSIS
Comments
#25 of United Kingdom writes: UK Screening and nitrogen storage requirements are only based on a single report with blood bank error which is a hugely different scale of cells involved from eggs, embryos and sperm. Segregation of screened samples is good - but with some common sense applied otherwise units will have many different dewars for each type of virus. All Consultant virologists aggree the risk of cross contamination in nitrogen for most gamete storage is ridiculously low.
 
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