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Survey: Outgassing IVF Plasticware
Background
Dr John Tyler of Next Generation Fertility, Sydney, Australia asks if clinics outgas their IVF tissue culture plasticware before use and if so how. Please add a comment on how you pre-process your dishes before adding media. What have you written in your policy manual? Has anyone performed studies on whether outgassing is needed?
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Survey has been filtered by All replies from all countries
The Question was:Do you outgas your plasticware. - Total yes replies= 27
1. The Option: Do you open the packet of plasticware and use it immediately to prepare your embryo dishes? [Results: Yes= 9 No: 18 Total= 27] ANALYSIS
2. The Option: Do you open the packet of plasticware and wait/ process the dishes before preparing your embryo dish [Results: Yes= 18 No: 9 Total= 27] ANALYSIS
The Question was:How do you process the dishes before use? - Total yes replies= 24
3. The Option: Do you expose the dishes to a sterile air flow ( e.g. in a laminar flow cabinet) [Results: Yes= 19 No: 5 Total= 24] ANALYSIS
4. The Option: Do you leave it in the incubator or equivalent? [Results: Yes= 1 No: 23 Total= 24] ANALYSIS
5. The Option: Do you wash the dishes in culture media or similar sterile solutions [Results: Yes= 4 No: 20 Total= 24] ANALYSIS
The Question was:If you outgas your dishes, for how long do you do it? - Total yes replies= 24
6. The Option: Less than 5 minutes [Results: Yes= 3 No: 21 Total= 24] ANALYSIS
7. The Option: 5-30 minutes [Results: Yes= 4 No: 20 Total= 24] ANALYSIS
8. The Option: 31-60 minutes [Results: Yes= 2 No: 22 Total= 24] ANALYSIS
9. The Option: More than 1 hour [Results: Yes= 1 No: 23 Total= 24] ANALYSIS
11. The Option: Overnight or longer [Results: Yes= 14 No: 10 Total= 24] ANALYSIS
The Question was:Do you replace the media in the dropletes before adding an oil overlay? - Total yes replies= 12
11. The Option: I replace the media in each drop with fresh media before adding the oil overlay? [Results: Yes= 7 No: 5 Total= 12] ANALYSIS
12. The Option: No I do not replace the media in each drop with fresh media before adding the oil overlay? I just add the drops of media then the oil. [Results: Yes= 5 No: 7 Total= 12] ANALYSIS
Comments
#56 of Australia writes: I would have thought outgassing basically any plastic consumables would be routine
#64 of USA writes: How do you process the dishes before use. Many of us place and remove our droplets and then replace with fresh media. I think this should be an added question.
 
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