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List of IVF Activities
The list of activities below include many of the common activities that currently required in an IVF Laboratory.
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Activity
Description
Discipline
Assisted Hatching
Either laser or Acid Digestion, cleavage or blastocyst stage, fresh or frozen embryos. Includes complete or partial hatching.
Embryology
Blastocyst culture
Correct identification of embryos, preparation of culture dishes,movement of embryos, correct grading of embryos for transfer, cryostorage or discard
Embryology
Embryo Transfer.
Correct identification of embryos, embryo grading, patient identification, loading of catheter and transfer of embryos to the patient.
Embryology
Fertilisation Check
Correct Identification, removal of granulosa material, correct identification of pronuclei and transfer of embryos to new culture media.
Embryology
ICSI
The correct identification of sperm and OCCC, the denuding of the oocyte, the set up of the injection equipment and the injection of the oocyte by a single spermatozoon.
Embryology
IVF Insemination
Correct identification of sperm and OCCC and their mixture
Embryology
Oocyte Collection
Identification of OCCC in follicular fluid and collection, washing and transfer to culture system
Embryology
Blastocyst Freezing
Correct patient identification and blastocyst grading,dehydration of embryos and loading into cryotools(straws), operation of freezer and movement to storage tanks. Completion of documentation.
CryoBiology
Blastocyst Thawing
Correct patient identification, location of blastocysts in storage tanks, rehydration of embryos, transfer to culture media. Documentation
CryoBiology
Blastocyst vitrification.
Correct patient identification, embryo grading of blastocysts, their vitrification and loading onto cryotools, and their movement to storage tanks. Documentation.
CryoBiology
Blastocyst Warming
Correct identification and warming of blastocysts, assessment of embryos, movement to culture media. Documentation
CryoBiology
Freezing of Cleavage Embryos
Correct patient identification, embryo grading of pronuclear or cleavage stage embryos, dehydration of embryos,loading into straws, operation of freezer and movement to storage tanks. Documentation.
CryoBiology
Oocyte Freezing
Correct identification and assessment of mature oocytes, dehydration, loading into straws,operation of freezer and movement to storage tanks. Documentation
CryoBiology
Oocyte Thawing
Correct patient identification, location of oocytes in storage tanks, their re-hydration, transfer to culture media. Documentation
CryoBiology
Oocyte Vitrification
Correct patient identification, grading of mature oocytes, their vitrification and loading onto cryotools, and their movement to storage tanks. Documentation.
CryoBiology
Oocyte Warming
Correct identification and warming of oocytes, their post-warm assessment, movement to culture media. Documentation
CryoBiology
Thawing of cleavage stage embryos
Correct patient identification, location of pronuclear or cleavage stage embryos in storage tanks, re-hydration of embryos, transfer to culture media. Documentation
CryoBiology
Vitrification of cleavage stage embryos
Correct patient identification, embryo grading of pronuclear or cleavage stage embryos, their vitrification and loading onto cryotools, and their movement to storage tanks. Documentation.
CryoBiology
Warming of Cleavage Stage Embryos
Correct identification and warming of pronuclear or cleavage stage embryos, assessment of embryos, movement to culture media. Documentation
CryoBiology
Blastocyst Biopsy
Zona breaching and removal of one or more trophectodermal cells and preparation for FISH or PCR.
PGD
Cleavage Stage Biopsy
Removal of one or more blastomeres of day 3 cleavage stage embryos and preparation for FISH or PCR.
PGD
Polar Body Biopsy
Removal of one or more polar bodies and preparation for FISH or PCR
PGD
ASAB
Assessment of Antisperm Antibodies by direct or indirect means- number of samples
Andrology
Semen Analysis
Receipt, identity check, volume, appearance, viscosity, liquefaction, pH, sperm concentration, motility and morphology estimation.
Andrology
Semen Analysis CASA
semen analysis including nay form of CASA device to estimate concentration, motility and/or morphology.
Andrology
Semen Cryopreservation
Freezing of a sperm sample by any means.
Andrology
Sperm DNA Fragmentation
Assessment of Sperm Chromatin by TUNEL, SCSA or other means
Andrology
Sperm Function Test
Assessment of sperm function by any means including reactive oxygen species, Zona or other binding studies, ARIC, hamster oocyte penetration.
Andrology
Sperm mucus Interaction
The assessment of sperm cervical mucus interaction by any means including post-coital or in vitro testing.
Andrology
Sperm preparation
Isolation of a motile fraction of spermatozoa from an ejaculate for IVF/ICSI/IUI/ by any means.
Andrology
Sperm Protamine Assessment
Assessment of Protamine/Chromatin Ratios by any means
Andrology
Surgical Collection
Examination of testicular or epididymal tissue and recovery of spermatozoa for freezing or direct insemination.
Andrology
MEA
Mouse embryo bioassay for the assessment of toxicity- number of episodes.
QC
Quality Activities (Hrs)
QC
Sperm Survival Studies
Assessment of toxicity by sperm survival test - number of episodes.
QC
Education & Training (hrs)
Private
Private
Individual Activity
Private
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